Study : Genome-wide identification of AGO18b-bound miRNAs and phasiRNAs in maize by cRIP-seq
Identification
Name
Genome-wide identification of AGO18b-bound miRNAs and phasiRNAs in maize by cRIP-seq
Identifier
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Source
Description
As a key member of the grass-specific subclade of argonaute proteins, AGO18b protein has been proposed to bind 24-nt meiotic phasiRNAs based on their co-expression in tapetal and meiotic cells. Here we show that expression of in maize tassels is strictly induced by developmental stages. Interestingly, reduced expression of AGO18b in both Mutator-mediated (ago18b::mum) and RNAi mutants leads to an increase in plant height and tassel length, suggesting its non-meiotic function as a negative regulator of SAM/IM maintenance. During the premiotic stage, AGO18b primarily binds to 21-nt phasiRNAs with 5’-uridine and less binds to 24-nt phasiRNAs with 5’-adenosine, coincident with its enrichment of miR2275 required for the 24-nt phasiRNA production. MiR166a, the negative regulator of SAM, is mostly enriched among AGO18b-bound miRNAs, implicating a novel negative regulator of IM. We suggest that AGO18b regulates maize tassel development via both phasiRNAs and miRNA pathways. Overall design: sRNA-Seq, mRNA-Seq and RIP-Seq for W22-ref and ago18b::mum samples, respectively. Both library type have two biological experiments. IgG samples were used as control for RIP-Seq. For RIP-Seq, we constructed two type libraries: short insertion for sRNAs and long insertion for mRNAs.
Genotype
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