Study : Fundamental molecular bases for differential transcriptional programs of top leaves during grain-filling in rice

Identification

• Name: Fundamental molecular bases for differential transcriptional programs of top leaves during grain-filling in rice
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTM1MDc5Mg==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Flag (FL) and second leaves (SL) in rice show differential aging patterns during monocarpic senescence. Coordination of aging programs in the top leaves is important for effective grain-filling. However, molecular bases for differential aging programs in the top leaves have not been systematically explored in rice. Here, we performed mRNA-sequencing of FL and SL at six time points during the grain-filling period. mRNA expression data revealed 6,365 genes showing aging-dependent expression changes in FL and/or SL. Of them, while 3047 genes showed shared aging-dependent expression patterns between FL and SL, 3058 genes showed differential expression patterns, which were classified into 5 major groups (G1-5) based on their differential expression patterns. Of the groups, G3 representing amino acid (AA) transport showed consistent differential age-dependent expression patterns in independent samples, whereas the other groups showed inconsistent differential expression patterns. Moreover, of AA transporters (AATs) in G3, long-distance AATs showed invariant differential age-dependent expression patterns after panicle removal, consistent to panicle removal-invariant differential nitrogen contents between FL and SL, known to be associated with protein concentration in grains. Our results suggest that long-distance AA transport is an invariant core transcriptional program of differential aging in rice top leaves for nitrogen remobilization during grain-filling. Overall design: mRNA expression profiles of the flag leaf and 2nd leaf of Oryza Sativa during grain filling (Day After Heading, DAH, 4-44) were examined by Illumina Hi-seq 2500.
• Data files: SRR4457253; SRR4457255; SRR4457256; SRR4457257; SRR4457258; SRR4457234; SRR4457236; SRR4457235; SRR4457237; SRR4457238; SRR4457239; SRR4457240; SRR4457241; SRR4457242; SRR4457243; SRR4457244; SRR4457245; SRR4457246; SRR4457247; SRR4457248; ERZ1140677; ERZ583543; ERZ1140763; ERZ583633; ERZ1140685; ERZ583510; ERZ1140776; ERZ583271; ERZ1140731; ERZ583330; ERZ1140751; ERZ583606; ERZ1140745; ERZ583589; ERZ1140733; ERZ583329; ERZ1140719; ERZ583303; ERZ1140699; ERZ583507