Study : Ulmus pumila cultivar:Jinye Transcriptome or Gene expression


Ulmus pumila cultivar:Jinye Transcriptome or Gene expression
We investigated the mechanism of greening of the yellow leaves of Jinye through high-throughput sequencing. Jinye samples were treated with different degrees of shade, to resolve the mechanism of changing leaf color. Our results provide a theoretical basis for the breeding of trees with colorful leaves. About 32.17 Gb clean data were obtained after the completion of sequencing, including 106,653,432 clean reads. The Q30 base percentage of four samples was not less than 92.08%. The sequencing data were assembled into 2,616,032 contigs and 62,945 unigenes. After comparing Ulmus pumila and other species to determine the unigenes in Ulmus pumila, 33,023 unigenes with annotated information were obtained. The NR (Non-Redundant) database showed the highest rate of notation at 48.93%, and the lowest was the COG (Cluster of Orthologous Groups) database at 19.05%. The number of differentially expressed genes (DEGs) increased and the proportion of upregulated genes decreased with increasing shade. The species distribution of the NR library showed that 7804 unigenes matched Morus notabilis, representing 25.35% homology. GO (Gene Ontology) enrichment results showed that different shading stresses corresponded to 58, 425, and 1301 differentially expressed unigenes annotated to the secondary structure of the GO library. GO enrichment results of DEGs under different stresses were similar. Comparison with the KEGG (Kyoto Encyclopedia of Genes and Genomes) library showed that the terpenoids and pigments of Ulmus pumila Jinye are strongly affected by light. This may be related to genes associated with pigment synthesis. This is the first report on the transcriptome of Ulmus pumila Jinye. Our sequencing results provide a basis for further research into this species.


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