Study : Setaria viridis cultivar:A10 Transcriptome or Gene expression
Identification
Name
Setaria viridis cultivar:A10 Transcriptome or Gene expression
Identifier
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Source
Description
Dichanthelium oligosanthes is small-genome C3 panicoid grass among a clade of predominately large genome C4 species. D. oligosanthes shares a common ancestor with Zea mays and Setaria viridis, both of which represent independent C4 lineages. Previous comparisons between C3 and C4 grasses have utilized C3 species from the BEP clade as outgroups, which diverged from the panicoid grass lineage over 50 million years ago. Thus, D. oligosanthes is ideally placed to serve as an in-group for panicoid comparative genomics. Here we report the assembly of the nuclear and chloroplast genomes of D. oligosanthes, as well as a comparative transcriptomics analysis of the developing leaf of D. oligosanthes, S. viridis, and Sorghum bicolor. The draft genome of D. oligosanthes was assembled using only high-throughput short read sequencing data. Physiological and anatomical characterizations verified that D. oligosanthes utilizes the C3 pathway for carbon fixation and lacks Kranz anatomy. Expression profiles of transcription factors (TFs) along developing leaves of D. oligosanthes, and S. viridis were compared with previously published data from S. bicolor, Z. mays and Oryza sativa to identify a small suite of TFs that likely acquired functions specifically related to C4 photosynthesis.The phylogenetic location of D. oligosanthes makes it an ideal C3 plant for comparative analysis of C4 evolution in the panicoid grasses. This genome will not only provide a better C3 in-group for comparisons of C4 grasses, but also highlights the power of high-throughput sequencing for addressing questions in evolutionary biology.
Genotype
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