Study : Transcriptome-based approach for gene discovery and metabolic engineering of camptothecin biosynthesis in Nothopodytes nimmoniana Graham.

Identification

• Name: Transcriptome-based approach for gene discovery and metabolic engineering of camptothecin biosynthesis in Nothopodytes nimmoniana Graham.
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTI5MzQwNg==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Nothapodytes nimmoniana (J.Graham) Mabb.(Icacinaceae),an important source of monoterpene indole alkaloid, camptothecin(CPT). Primary cellular target of CPT is DNA topoisomerase I (topo I). However, number of reports have indicated its therapeutic potential against colon cancer , uterine cervical cancer, ovarian cancer, AIDS and malaria . Two clinically used anti-tumor compounds, topotecan and irinotecan, are currently semi-synthesized using natural camptothecins. Biosynthetic pathway of CPT is yet to be fully enunciated. Transcriptome analysis offers a powerful platform for pathway elucidation and determining the expression profile of genes involved in CPT biosynthesis at the molecular level.
• Data files: SRR2310724; SRR2310885