Study : Transcriptome profiling of the defense response following elicitation by a bacterial pathogen or flg22

Identification

• Name: Transcriptome profiling of the defense response following elicitation by a bacterial pathogen or flg22
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTI4Njc2OA==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Overall design: Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.
• Data files: SRR2060318; SRR2060319; SRR2060320; SRR2060321; SRR2060322; SRR2060323; SRR2060324; SRR2060325; SRR2060326; SRR2060327; SRR2060328; SRR2060329