Study : The α-gliadin immunogenic complex in wheat

Identification

• Name: The α-gliadin immunogenic complex in wheat
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTI3MDk3NQ==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: The α-gliadins present in wheat flour contain the most-immunogenic epitopes which are responsible for gluten intolerances. Previous studies on wheat were focused on individual epitopes but not on the protein fragments containing those epitopes. In this work a comprehensive study of the main fragment of α-gliadins containing immunogenic epitopes has been carried out. By using NGS technology, six types of α-gliadins were identified in diploid and polyploid wheats. We show that Type 1 α-gliadins, but not the other types, contain all the immunogenic peptides and epitopes. This Type 1 sequence underwent two independent rounds of small insertion events in the α-gliadin repetitive region giving rise to the most-toxic 33-mer peptide containing six overlapping epitopes in bread (hexaploid) wheat. These findings are important for CD and wheat breeding as we identify five types of α-gliadins that do not contain toxic epitopes.
• Data files: SRR1732323; SRR1732325; SRR1732368; SRR1732369; SRR1732378; SRR1732379; SRR1732380; SRR1732381; SRR1732382; SRR1732383; SRR1732384; SRR1732385; SRR1732386; SRR1732387; SRR1732388; SRR1732389; SRR1732390; SRR1732391; SRR1732392; SRR1732393