Study : Arabidopsis thaliana Transcriptome or Gene expression
Arabidopsis thaliana Transcriptome or Gene expression
Alternative splicing is prevalent in plants, but little is known about its regulation in the context of developmental and signaling pathways. In a genetic screen, we identified a new factor, AtRTF2, that influences pre-mRNA splicing and is essential for embryonic development in Arabidopsis thaliana. A genome-wide analysis using RNA-seq indicated that 12% of total introns are retained to a significant degree in atrtf2 mutants. Based on yeast two-hybrid and affinity purification-mass spec analysis, we suggest that AtRTF2 may act in ubiquitin-based regulation of pre-mRNA splicing and membrane signaling to the spliceosome.