Study : Global Changes following N-deprivation and N-resupply in Chlamydomonas in the cht7 mutant and the wild-type: Illumina sequencing

Identification

Name

Global Changes following N-deprivation and N-resupply in Chlamydomonas in the cht7 mutant and the wild-type: Illumina sequencing

Identifier

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Source

Data link

Link to this study on EBI European Nucleotide Archive

Description

Responding to environmental cues such as nutrient deprivation, cells can halt divisions and enter quiescence, which unlike terminal cell fates is reversible. Drastic changes in cell metabolic status accompany the entry and exit of quiescence and specific regulatory mechanisms that integrate metabolism with the cell division cycle are largely unknown. Here, we describe the identification of “Compromised Hydrolysis of Triacylglycerols 7” (CHT7), a protein of Chlamydomonas reinhardtii with predicted CXC DNA-binding domains. The cht7 mutant accumulates triacylglycerols during N deprivation-induced quiescence, but fails to degrade triacylglycerols after N refeeding and cannot exit quiescence even though cells are viable. Global transcript analysis suggests dual roles for CHT7 as it appears to safeguard against spontaneous quiescence entry during N-replete growth and is required to activate metabolic genes during quiescence exit. CHT7 is a component of a multiprotein complex that appears to adjust specific transcriptional subnetworks at quiescence entry and exit.

Data files

Genotype

Accession number	Name	Taxon