Study : Brachypodium distachyon Transcriptome or Gene expression

Identification

• Name: Brachypodium distachyon Transcriptome or Gene expression
• Identifier: dXJuOkVWQS9zdHVkeS9QUkpOQTEyNTY5Nw==
• Data link: Link to this study on EBI European Nucleotide Archive
• Description: Deep sequencing of Brachypodium distachyon small RNA from panicles (flowers) was done to analyze the genome-wide distribution patterns of 1) total small RNA reads and loci, 2) 21 and 24 nucleotide repeat-normalized reads and 3) 21 and 24 nucleotide phased siRNA clusters relative to gene and transposable element density. Overall design: Small RNA were extracted from total RNA by size fractionation and converted to DNA amplicons by serial adaptor ligation to both ends followed by RT-PCR. DNA amplicons were sequenced using an Ilumina Genome Analyzer. Resulting sequences were computationally trimmed to remove 3 adaptor sequences. Raw data for GSM506621 was not provided.
• Data files: SRR035616