Study : Zea mays Transcriptome or Gene expression


Zea mays Transcriptome or Gene expression
This research identifies a novel protein required for paramutation at the maize purple plant1 locus. This required to maintain repression2 (RMR2) protein represents the founding member of a plant-specific clade of hypothetical proteins. We show that RMR2 is required for transcriptional repression at the Pl1-Rhoades haplotype, for accumulation of 24 nt RNA species, and for maintenance of a 5-methylcytosine pattern distinct from that maintained by RNA polymerase IV. Genetic tests indicate that RMR2 is not required for paramutation occurring at the red1 locus. These results distinguish the paramutation-type mechanisms operating at specific haplotypes. The RMR2 clade of proteins provides a new entry point for understanding the diversity of epigenomic control operating in higher plants. Overall design: Examination of small RNAs using Illuminas sequencing-by-synthesis (SBS) platform to deep sequence small RNA libraries made from the 4-cm cobs of rmr2 mutant and non-mutant siblings.
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Accession number Name Taxon