Study : We exome captured and sequenced 397 diverse barley accessions to carry out a genome-wide analysis copy number variants affecting coding sequences. The Roche Nimblegen exome capture array was used to enrich for exon sequences (http://sequencing.roche.com/products/nimblegen-seqcap-target-enrichment/seqcap-ez-system/seqcap-ez-designs.html). The enriched samples were then sequenced using an Illumina HiSeq 2000 sequencer. An average of 2 x 24.6 million paired end Illumina reads per sample were generated, which correspond to approximately 80x coverage of the 61.3 Mbp exome capture space.
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We exome captured and sequenced 397 diverse barley accessions to carry out a genome-wide analysis copy number variants affecting coding sequences. The Roche Nimblegen exome capture array was used to enrich for exon sequences (http://sequencing.roche.com/products/nimblegen-seqcap-target-enrichment/seqcap-ez-system/seqcap-ez-designs.html). The enriched samples were then sequenced using an Illumina HiSeq 2000 sequencer. An average of 2 x 24.6 million paired end Illumina reads per sample were generated, which correspond to approximately 80x coverage of the 61.3 Mbp exome capture space.
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