Study : transcriptome analysis for haustroium development in Cuscuta campestris parasitizing on host plant A. thaliana
Identification
Name
transcriptome analysis for haustroium development in Cuscuta campestris parasitizing on host plant A. thaliana
Identifier
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Description
The genus Cuscuta are stem parasitic angiosperms that parasitize a wide range of vascular plants via de novo formation of a distinctive parasitic organ called haustorium. In the developing haustorium, meristematic cells, which are initiated from the stem cortical tissue, differentiate into haustorial parenchyma cells, which elongate, penetrate into the host tissues and finally connect with the host vasculature. This interspecific vasculature-connection allows the parasite to uptake water and nutrients from the host plant. Although histological aspects of the haustorial development have been studied extensively, the molecular mechanisms underlying the vasculature development and the interspecific connection with the host vasculature still remain mostly unknown. To gain insight into the interspecific cell to cell interaction in the haustorium development, we established an in vitro haustorium induction system for Cuscuta campestris using Arabidopsis thaliana rosette leaves as the host plant tissue. Using the in vitro induction system, we showed involvement of host tissue for the differentiation of haustorial cells into xylem vessel cells. To characterize molecular event during the host-dependent xylem vessel cell differentiation in C. campestris, we carried out a transcriptome analysis using the in vitro induction system. The data showed that orthologs of a set of genes known to be involved in development and proliferation of vascular stem cell were up-regulated without the presence of the host tissue, whereas the expression of orthologs known to be required for xylem vessel cell differentiation were up-regulated after some haustorial cells elongated and reached to the host xylem. This result was supported by another transcriptome analysis for the haustroium development in intact C. campestris parasitizing on the host plant. These findings suggest involvement of host-derived signals in the regulation of non-autonomous xylem vessel differentiation and its connection to the host vasculature during the haustorium development by activating a set of key genes for differentiation into xylem vessel cell.
Genotype
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