Study : Investigation of the response to DNA damage in the moss Physcomitrella patens
Identification
Name
Investigation of the response to DNA damage in the moss Physcomitrella patens
Identifier
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Source
Description
DNA in living organisms is frequently damaged by cellular activities such as replication and transcription and by severe conditions such as high reactive oxygen species levels, low temperatures, UV, and heavy metals. To investigate the responses of land plants to DNA damage, we analyzed the transcriptome of the moss Physcomitrella patens with or without DNA damage. In detail, leafy shoots of Physcomitrella (known as gametophores) were treated for 6 hours with or without Zeocin, an inducer of DNA strand breaks, and transcriptome analysis was performed during, after, and in the absence of Zeocin treatment. Changes in the transcriptome were analyzed to elucidate the trajectory of cell-state changes induced by DNA strand breaks, and changes in the transcript level of each gene were analyzed to identify candidate genes involved in the response to DNA strand breaks.
Data files
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