Study : Deep sequencing of small RNAs in grass-clump dwarf phenotype of abnormal growth triploids in interspecific crosses between tetraploid wheat and Aegilops tauschii
Identification
Name
Deep sequencing of small RNAs in grass-clump dwarf phenotype of abnormal growth triploids in interspecific crosses between tetraploid wheat and Aegilops tauschii
Identifier
dXJuOkVWQS9zdHVkeS9QUkpEQjQ2NjM=
Description
Triploid wheat hybrids between tetraploid wheat and Aegilops tauschii sometimes show abnormal growth phenotypes, and the growth abnormalities inhibit generation of wheat synthetic hexaploids. In type II necrosis, one of the growth abnormalities, necrotic cell death accompanied by marked growth repression occurs only under low temperature conditions. At normal temperature, the type II necrosis lines show grass-clump dwarfism with no necrotic symptoms, excess tillers, more severe dwarfism or delayed flowering. We compared small RNA expression profiles in crown tissues to characterize the temperature-dependent phenotypic plasticity. Some microRNAs, including miR156, were up-regulated, whereas the levels of transcripts of the miR156 target genes SPLs, known to inhibit tiller and branch number, were reduced in crown tissues of the grass-clump dwarf lines at the normal temperature. Dramatic alteration of gene expression profiles, including miRNA levels, in crown tissues is associated with the temperature-dependent phenotypic plasticity in type II necrosis/grass-clump dwarf wheat hybrids.
Genotype
Accession number | Name | Taxon |
---|