Study : Comparative transcriptomic analysis of max2, hxk1 mutant and Wild type seedlings treated with glucose in early seedling development
Identification
Name
Comparative transcriptomic analysis of max2, hxk1 mutant and Wild type seedlings treated with glucose in early seedling development
Identifier
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Description
A. thaliana seeds of max2, hxk1, and WT were surface-sterilized and allowed to imbibe water in the dark at 4 ?C for 3 d. The seeds were then transferred to Petri dishes containing water with 2% glucose or mannitol. The cultures were shaken at 140 rpm at 23 ?C for 3d under fluorescent white light with a 16 h light/8 h dark photoperiod.The seedlings were collected and rinsed three times with sterile distilled water, and excess water was removed by centrifugation. Total RNA was extracted from the seedlings and conducted a comparative transcriptomic analysis using the short-read sequencing technology (Illumina) combined with a tag-based digital gene expression (DGE) system.
Genotype
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