Study : Correction and analysis of full length cDNA from Sorghum bicolor BTx623
Correction and analysis of full length cDNA from Sorghum bicolor BTx623
To provide a resource of comprehensive expressed sequence data of Sorghum bicolor BTx623 and facilitate finding novel transcription units and accurate structural gene annotation, we constructed a normalized full length-cDNA library from aerial tissues at eight growth stages using the CAP trapper method and isolated 37,607 clones. These clones were Sanger sequenced from both the 5 and 3 ends and in total 38,981 high quality expressed sequence tags (ESTs) were obtained.The plants used for RNA extraction were grown in soil pots in greenhouse. Tissues were collected from plants at eight developmental stages from a week until 5 months after sawing. Total RNAs form each tissues were extracted using SDS/phenol method followed by LiCl purification and was mixed and used for making cDNA library.