BES for 5AS and 5AL chromosome About header nomenclature: for example: Tae5AL117L20FOR1 is 5' end of the clone no 117L20 of 5AL library; when there are situation like this: Tae5AL117L20FOR1 and Tae5AL117L20FOR2, it means that the sequencing was repeated twice. "Tae" means Triticum aestivum and it corresponds to "TaaCsp" in the clone name; for example: Tae5AL117L20FOR1 corresponds to clone no TaaCsp5AL117L20 (in the excell tables) other information... The BAC clones are positioned at the ends of contigs identified in the chromosome 5A FPC-MTPs were recovered and BESs (5' and 3') were obtained with the Sanger technology. The total number of raw BESs was 5473 and 6412 for 5AS and 5AL, respectively. Raw BESs were subsequently quality filtered for presence of nondetermined nucleotides, duplications, extreme biases in base composition, and length below 50 bp. This resulted in 3861 and 5194 final high-quality BESs for 5AS and 5AL, respectively. The BESs were screened computationally to develop a panel of TE-based markers [ISBP] and to design SSR. In 5AS, 896 BESs carried at least one ISBP (corresponding to 791 BAC clones or 31.8% of the sequenced BAC), with a maximum of seven possible ISBPs in the same sequence, while for 5AL, 1068 BESs had at least one ISBP (961 BACs or 33.9% of the sequenced BAC), with a maximum of seven ISBPs per sequence, as well. Moreover, 124 SSRs were identified with the Imperfect SSR Finder program, 72 for 5AS and 52 for 5AL, including di-, tri-, and tetranucleotide repetitions (5AS: 34 di-, 37 tri-, 1 tetra-; 5AL: 32 di-, 20 tri-). A total of 2086 BESs (around 25% of the sequences produced) had at least one potentially useful molecular marker. The BESs were in silico anchored to: - genes of genome zipper (Brachypodium) throughout BLASTn search where similarity E-value cutoff lower than 1x 10-10 and an identity higher than 95% were applied. No more than six mismatches were allowed. In this way the contigs in which the BES are included were linked for inference to the BLASTed genes, that were mapped to the physical map. - the IWGSC chromosome survey sequences (CSSs). BLASTn searches (E-value > 1 x 10-10) were performed, a minimum similarity of 600 bp, at least 99% sequence identity, and no more than five mismatches were considered. All these results are reported in the attached tables.