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# T. aestivum chromosome 2A BAC end sequences (BES) #
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LIBRARY INFORMATION
Library code:   TaaCsp2ALhA (Long Arm), TaaCsp2AShA (Short Arm)	
Class:          BAC ends
Organism:       Triticum aestivum
Cultivar:       Chinese Spring
Vector:         pIndigoBAC
R. Site 1:      HindIII
R. Site 2:      HindIII
Description:    see Safar et al. (2004) Dissecting large and complex
                genomes: flow sorting and BAC cloning of individual
                chromosomes from bread wheat. Plant J., 39, 960-8.


2A_ShortArm_BES.fasta 
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Format:              		FASTA
Type (of 1st seq):   		DNA
Number of sequences: 		98488
Total number of residues:	73054535
Smallest:            		200
Largest:             		1120
Average length:      		741.8

2A_LongArm_BES.fasta 
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Format:              		FASTA
Type (of 1st seq):   		DNA
Number of sequences: 		135164
Total number of residues:	97554970
Smallest:            		200
Largest:             		1092
Average length:      		721.8



BAC end sequencing was done using T7 (forward Primer) and RP1 (Reverse Primer)from both the directions. Capillary electrophoresis of the sequencing reaction was carried out with a 3730xl DNA Analyzer (Applied Biosystems, Foster City, CA).All the 2A BAC end sequence chromatogram files were base called and trimmed using Phred program (Phred score 20). Default value (0.05) was used for Phred trimming with option "trim_alt" to carry out trimming based on Phred quality value of each base. CROSS_MATCH (version 0.990329) software was used to mask the sequence of pIndigoBAC-5 BAC vector.