Spads
- Version: 1.2.0
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- Spads
Spads
Specific Primers & Amplicon Design Software
FUNCTION
GENOPLANTETMSPADS tries to design a unique GST (Gene-Sequence Tag) and a specific primer set for its PCR amplification, on a genomic sequence, knowing the gene structure.
DESCRIPTION
In transcriptome approaches, probes corresponding to individual members of multigenic families often lack specificity and are responsible of molecular cross-hybridization events. GENOPLANTETMSPADS uses a strategy to optimize the selection of specific probes or unique GST (Gene Sequence Tag) in a poor conserved region of each gene in a given genome. It can be applied when the genome considered is fully sequenced or if the gene family involved is exhaustively characterized. From a gene for which the intron-exon structure is known (or predicted), the program selects, by comparison with the whole genome, the less conserved region(s), and designs a primer pair specific to that region for its amplification from genomic DNA.
Access mode(s):
Web free accessCommand line on saruman.versailles.inra.fr
Keyword(s):
primer PCR amplification genome sequenceCreation date: 14 Oct 2011
eZ PublishPublication supervisor: A-F. Adam-Blondon
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