Protein : Qrob_P0563560.2 Q. robur
Protein Identifier  ? Qrob_P0563560.2 Organism . Name  Quercus robur
Score  98.0 Score Type  egn
Protein Description  (M=2) K04485 - DNA repair protein RadA/Sms Code Enzyme  EC:3.6.1.8
Gene Prediction Quality  validated Protein length 

Sequence

Length: 348  
Kegg Orthology  K04485
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003677 DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0003684 damaged DNA binding Interacting selectively and non-covalently with damaged DNA.
GO:0006281 DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
GO:0008094 DNA-dependent ATPase activity Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of single- or double-stranded DNA, and it drives another reaction.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cit:102628276 1 315 + 315 Gaps:26 57.46 583 60.00 3e-125 uncharacterized LOC102628276
blastp_kegg lcl|cic:CICLE_v10025165mg 1 315 + 315 Gaps:28 53.86 622 60.30 2e-124 hypothetical protein
blastp_kegg lcl|vvi:100258398 6 316 + 311 Gaps:76 60.74 624 53.30 2e-115 DNA repair protein RadA homolog
blastp_kegg lcl|pop:POPTR_0001s19200g 88 316 + 229 Gaps:10 37.40 639 71.13 6e-110 POPTRDRAFT_1067679 hypothetical protein
blastp_kegg lcl|tcc:TCM_028919 65 316 + 252 Gaps:11 41.42 635 66.54 9e-109 ATP-dependent peptidases nucleotide binding serine-type endopeptidases DNA helicases ATP binding damaged DNA binding nucleoside-triphosphatases isoform 1
blastp_kegg lcl|sot:102603127 1 316 + 316 Gaps:40 58.35 593 53.76 4e-108 uncharacterized LOC102603127
blastp_kegg lcl|sly:101253170 1 316 + 316 Gaps:40 58.35 593 53.76 3e-106 DNA repair protein RadA homolog
blastp_kegg lcl|csv:101217616 1 316 + 316 Gaps:62 58.73 630 51.62 2e-104 DNA repair protein RadA homolog
blastp_kegg lcl|cmo:103489983 70 316 + 247 Gaps:3 38.36 636 66.39 4e-103 uncharacterized LOC103489983
blastp_kegg lcl|fve:101299495 1 316 + 316 Gaps:57 59.14 607 53.20 1e-102 DNA repair protein RadA homolog
blastp_uniprot_sprot sp|Q9KGG1|RADA_BACHD 91 316 + 226 Gaps:15 46.61 457 42.72 2e-46 DNA repair protein RadA homolog OS Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|Q92F42|RADA_LISIN 90 316 + 227 Gaps:15 46.39 457 42.45 2e-45 DNA repair protein RadA homolog OS Listeria innocua serovar 6a (strain CLIP 11262) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|Q48761|RADA_LISMO 90 316 + 227 Gaps:15 46.39 457 41.98 5e-45 DNA repair protein RadA homolog OS Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) GN radA PE 3 SV 2
blastp_uniprot_sprot sp|P37572|RADA_BACSU 91 316 + 226 Gaps:24 46.72 458 41.59 3e-39 DNA repair protein RadA homolog OS Bacillus subtilis (strain 168) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|P45266|RADA_HAEIN 94 316 + 223 Gaps:12 46.07 458 39.81 7e-39 DNA repair protein RadA homolog OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|Q8P2Q5|RADA_STRP8 91 316 + 226 Gaps:20 46.36 453 39.05 2e-38 DNA repair protein RadA homolog OS Streptococcus pyogenes serotype M18 (strain MGAS8232) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|Q5XDZ7|RADA_STRP6 91 316 + 226 Gaps:20 46.36 453 39.05 2e-38 DNA repair protein RadA homolog OS Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|Q9A1K1|RADA_STRP1 91 316 + 226 Gaps:20 46.36 453 39.05 2e-38 DNA repair protein RadA homolog OS Streptococcus pyogenes serotype M1 GN radA PE 3 SV 1
blastp_uniprot_sprot sp|P74391|RADA_SYNY3 92 316 + 225 Gaps:34 47.33 505 40.17 2e-38 DNA repair protein RadA homolog OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN radA PE 3 SV 1
blastp_uniprot_sprot sp|P96963|RADA_PSEAE 92 316 + 225 Gaps:15 46.36 453 40.48 3e-38 DNA repair protein RadA homolog OS Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN radA PE 3 SV 2
rpsblast_cdd gnl|CDD|183326 92 316 + 225 Gaps:23 45.29 446 45.05 9e-67 PRK11823 PRK11823 DNA repair protein RadA Provisional.
rpsblast_cdd gnl|CDD|29987 97 317 + 221 Gaps:14 55.65 372 47.34 6e-63 cd01121 Sms Sms (bacterial radA) DNA repair protein. This protein is not related to archael radA any more than is to other RecA-like NTPases. Sms has a role in recombination and recombinational repair and is responsible for the stabilization or processing of branched DNA molecules..
rpsblast_cdd gnl|CDD|31265 92 317 + 226 Gaps:13 47.15 456 40.47 4e-54 COG1066 Sms Predicted ATP-dependent serine protease [Posttranslational modification protein turnover chaperones].
rpsblast_cdd gnl|CDD|161868 92 316 + 225 Gaps:15 47.58 454 40.74 1e-47 TIGR00416 sms DNA repair protein RadA. The gene protuct codes for a probable ATP-dependent protease involved in both DNA repair and degradation of proteins peptides glycopeptides. Also known as sms. Residues 11-28 of the SEED alignment contain a putative Zn binding domain. Residues 110-117 of the seed contain a putative ATP binding site both documented in Haemophilus and in Listeria monocytogenes for E.coli see ( J. BACTERIOL. 178:5045-5048(1996)).
rpsblast_cdd gnl|CDD|205659 146 317 + 172 Gaps:41 96.39 194 24.06 6e-15 pfam13481 AAA_25 AAA domain. This AAA domain is found in a wide variety of presumed DNA repair proteins.
rpsblast_cdd gnl|CDD|29986 189 332 + 144 Gaps:15 90.30 165 26.85 8e-13 cd01120 RecA-like_NTPases RecA-like NTPases. This family includes the NTP binding domain of F1 and V1 H+ATPases DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. This group also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion..
rpsblast_cdd gnl|CDD|29985 180 317 + 138 Gaps:39 68.18 242 24.24 2e-11 cd00984 DnaB_C DnaB helicase C terminal domain. The hexameric helicase DnaB unwinds the DNA duplex at the chromosome replication fork. Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown a change in the quaternary structure of the protein involving dimerization of the N-terminal domain has been observed and may occur during the enzymatic cycle. This C-terminal domain contains an ATP-binding site and is therefore probably the site of ATP hydrolysis..
rpsblast_cdd gnl|CDD|30653 182 314 + 133 Gaps:39 36.78 435 25.62 7e-10 COG0305 DnaB Replicative DNA helicase [DNA replication recombination and repair].
rpsblast_cdd gnl|CDD|197690 186 317 + 132 Gaps:9 83.11 148 23.58 1e-09 smart00382 AAA ATPases associated with a variety of cellular activities. AAA - ATPases associated with a variety of cellular activities. This profile/alignment only detects a fraction of this vast family. The poorly conserved N-terminal helix is missing from the alignment.
rpsblast_cdd gnl|CDD|30815 176 280 + 105 Gaps:7 37.69 260 30.61 2e-09 COG0467 RAD55 RecA-superfamily ATPases implicated in signal transduction [Signal transduction mechanisms].

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 64 316 253 PTHR32472 none none none
PANTHER 64 316 253 PTHR32472:SF10 none none none
PRINTS 99 123 25 PR01874 none DNA repair protein radA signature IPR004504
PRINTS 178 206 29 PR01874 none DNA repair protein radA signature IPR004504
PRINTS 275 299 25 PR01874 none DNA repair protein radA signature IPR004504
SUPERFAMILY 177 319 143 SSF52540 none none IPR027417
Pfam 176 317 142 PF13481 none AAA domain none
Gene3D 178 317 140 G3DSA:3.40.50.300 none none IPR027417
ProSiteProfiles 166 320 155 PS50162 none RecA family profile 1. IPR020588
SMART 186 345 160 SM00382 none ATPases associated with a variety of cellular activities IPR003593

0 Localization

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC_3P Qrob_Chr05 5 s_2GDU0O_517 s_1BT8O3_239 25,81 0 55,45 lod 2,2101 5,5

0 Targeting